RNA interference, a natural mechanism that inhibits the gene expression of individual genes in eukaryotic cells, is a major topic in modern biology. However, their potential was usable to only a ...

The discovery of siRNA and its role in RNAi was a groundbreaking finding in the field of molecular biology. In 1998, Andrew Fire and Craig Mello published a seminal paper describing the phenomenon of ...

Cell division is the generative spark of nearly every lifeform on Earth. But if healthy growth goes unchecked, cell division can turn lethal, overwhelming the organism. Such is the case with so-called ...

Gene silencing refers to any mechanism that turns down or switches off the expression of a specific gene so that less of its protein, or none at all, is produced. Crucially, the gene's DNA is left ...

In a study published in the Clinical and Translational Medicine, a Nanjing University research group evaluated the on- and off-target effects of self-assembled siRNA delivery system. RNA interference ...

Figure 1: Design, preparation, and therapeutic mechanisms of PPCi NPs. (A) Self-assembly process of PPCi NPs. The opposite electrical properties of PEG- PEI (positively charged) and siRNA (negatively ...

This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway will be ...