Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...

What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

A project at Swiss research center EPFL has designed an add-on unit intended to bring structured light capability to a standard optical microscope. Described in Nature Communications, the design ...

For hundreds of years, the optical microscope was the only tool available to scientists wanting to study the movement of cells, bacteria and yeast. But the diffraction of light made it impossible to ...

SIMIP enables high-resolution images rich in both chemical and spatial information. A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator (SLM) generates striped ...

Researchers used structured light and switchable fluorescent molecules to reduce the background light from the out-of-plane regions of microscope samples. This method allowed for the acquisition of ...

Existing super-resolution techniques still face considerable challenges in complex deep tissue environments. For example, Stimulated Emission Depletion (STED) microscopy relies on a doughnut-shaped ...

This is especially true when studying intricate details beyond the optical resolution limit using structured illumination microscopy (SIM). SIM uses multiple images acquired under structured ...

Laser-induced fluorescence (LIF) thermometry has emerged as a robust and non-intrusive approach for mapping temperature distributions in complex spray environments. By utilising laser excitation to ...

Cryogenic microscopy at Diamond Light Source enables high-resolution, correlative imaging of cells under near-physiological ...