Nature

Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases

When used to edit genomes, Cas9 nucleases produce targeted double-strand breaks in DNA. Subsequent DNA-repair pathways can induce large genomic deletions (larger than 100 bp), which constrains the ...
Nature

Robust prediction of synthetic gRNA activity and cryptic DNA repair by disentangling cellular CRISPR cleavage outcomes

The alternative text for this image may have been generated using AI. Here, we disentangle cellular CRISPR–Cas9 cleavage outcomes in human cells after cleavage by synthetic gRNA and reveal sometimes ...